The determination of number-, weight-, and z-average molecular weights by meniscus-depletion sedimentation equilibrium
نویسندگان
چکیده
منابع مشابه
Determination of membrane protein molecular weights and association equilibrium constants using sedimentation equilibrium and sedimentation velocity.
Regulated molecular interactions are essential for cellular function and viability, and both homo- and hetero-interactions between all types of biomolecules play important cellular roles. This chapter focuses on interactions between membrane proteins. Knowing both the stoichiometries and stabilities of these interactions in hydrophobic environments is a prerequisite for understanding how this c...
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A method is presented by which an experimental record of total concentration as a function of radial distance, obtained in a sedimentation equilibrium experiment conducted with a noninteracting mixture in the absence of a density gradient, may be analyzed to obtain the unimodal distributions of molecular weight and of partial molar volume when these vary concomitantly and continuously. Particul...
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The discovery that purified protein preparations consist of molecules which are monodisperse with respect to molecular weight was originally entirely unexpected (1). Polymerization processes in general are expected to yield products which contain molecules with a wide range of molecular size, and only a template mechanism of synthesis can reasonably be expected to yield size homogeneity. It is ...
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The problem of obtaining meaningful molecular weights for high molecular weight DNA samples by sedimentation equilibrium in a density gradient has been solved by correcting the theory for thermodynamio non-ideality. Several different methods of virial corrections are suggested and examined to determine the best method of obtaining molecular weights at infinite dilution. A pronounced virial effe...
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In this paper we discuss a technique for calculating moments of polydisperse materials in terms of concentration readings along the cell. The proposed method minimizes dependence on data from the en'd points ·where they may be unreliable'. An analysis is given of the errors involved in the use of the proposed method when the underlying molecular weight distribution is the Schulz distribution or...
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ژورنال
عنوان ژورنال: Journal of Polymer Science Part B: Polymer Physics
سال: 1988
ISSN: 0887-6266,1099-0488
DOI: 10.1002/polb.1988.090260518